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human skin fibroblast cdna library  (TaKaRa)


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    Structured Review

    TaKaRa human skin fibroblast cdna library
    Human Skin Fibroblast Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 543 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skin+fibroblast+cdna+library/Human+Brain%2C+Whole+QUICK-Clone+cDNA/pm33580598-39-30-35
    Average 94 stars, based on 543 article reviews
    human skin fibroblast cdna library - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Library Screening:

    Article Title: NFI-B3, a novel transcriptional repressor of the nuclear factor I family, is generated by alternative RNA processing.
    Article Snippet: .. Library Screening and DNA Sequencing—A commercially available human skin fibroblast cDNA library (Clontech HL 1052a) was screened with a previously isolated 1.2-kb NFI-X cDNA clone, FN6, under hybridization conditions as described (9). ..

    Sequencing:

    Article Title: NFI-B3, a novel transcriptional repressor of the nuclear factor I family, is generated by alternative RNA processing.
    Article Snippet: .. Library Screening and DNA Sequencing—A commercially available human skin fibroblast cDNA library (Clontech HL 1052a) was screened with a previously isolated 1.2-kb NFI-X cDNA clone, FN6, under hybridization conditions as described (9). ..

    Article Title: CASH, a Novel Caspase Homologue with Death Effector Domains
    Article Snippet: .. To obtain the full-length cDNA of CASH, we screened human skin fibroblast cDNA library (CLONTECH) with a cDNA probe corresponding to the CASH sequence. ..

    cDNA Library Assay:

    Article Title: NFI-B3, a novel transcriptional repressor of the nuclear factor I family, is generated by alternative RNA processing.
    Article Snippet: .. Library Screening and DNA Sequencing—A commercially available human skin fibroblast cDNA library (Clontech HL 1052a) was screened with a previously isolated 1.2-kb NFI-X cDNA clone, FN6, under hybridization conditions as described (9). ..

    Article Title: Novel strategies for increased copy number and expression of recombinant human gelatin in Pichia pastoris with two antibiotic markers
    Article Snippet: Novel strain development pastoris.. Strains were first To determine if use of an increase copy number, the strain containing a differe that expressed both gelati cassettes.. Quantitative eva strated that increased exp both methods using two a

    Article Title: CASH, a Novel Caspase Homologue with Death Effector Domains
    Article Snippet: .. To obtain the full-length cDNA of CASH, we screened human skin fibroblast cDNA library (CLONTECH) with a cDNA probe corresponding to the CASH sequence. ..

    Article Title: Gelatin expression from an engineered Saccharomyces cerevisiae CUP1 promoter in Pichia pastoris.
    Article Snippet: Funding information FibroGen, Inc Abstract The methylotrophic yeast Pichia pastoris (reclassified as Komagataella phaffii) is a versatile protein expression system, yet many commonly used promoters have attributes undesirable for fermentation or its optimization.. Hence, the copper-inducible CUP1 gene promoter from the related yeast Saccharomyces cerevisiae was used to express human gelatin.. Multimerization of a potential copper response element in the CUP1 promoter, a S. cerevisiae Ace1p binding site, significantly increased gelatin expression.

    Isolation:

    Article Title: NFI-B3, a novel transcriptional repressor of the nuclear factor I family, is generated by alternative RNA processing.
    Article Snippet: .. Library Screening and DNA Sequencing—A commercially available human skin fibroblast cDNA library (Clontech HL 1052a) was screened with a previously isolated 1.2-kb NFI-X cDNA clone, FN6, under hybridization conditions as described (9). ..

    Hybridization:

    Article Title: NFI-B3, a novel transcriptional repressor of the nuclear factor I family, is generated by alternative RNA processing.
    Article Snippet: .. Library Screening and DNA Sequencing—A commercially available human skin fibroblast cDNA library (Clontech HL 1052a) was screened with a previously isolated 1.2-kb NFI-X cDNA clone, FN6, under hybridization conditions as described (9). ..

    Amplification:

    Article Title: Novel strategies for increased copy number and expression of recombinant human gelatin in Pichia pastoris with two antibiotic markers
    Article Snippet: Novel strain development pastoris.. Strains were first To determine if use of an increase copy number, the strain containing a differe that expressed both gelati cassettes.. Quantitative eva strated that increased exp both methods using two a

    Article Title: Gelatin expression from an engineered Saccharomyces cerevisiae CUP1 promoter in Pichia pastoris.
    Article Snippet: Funding information FibroGen, Inc Abstract The methylotrophic yeast Pichia pastoris (reclassified as Komagataella phaffii) is a versatile protein expression system, yet many commonly used promoters have attributes undesirable for fermentation or its optimization.. Hence, the copper-inducible CUP1 gene promoter from the related yeast Saccharomyces cerevisiae was used to express human gelatin.. Multimerization of a potential copper response element in the CUP1 promoter, a S. cerevisiae Ace1p binding site, significantly increased gelatin expression.

    Polymerase Chain Reaction:

    Article Title: Novel strategies for increased copy number and expression of recombinant human gelatin in Pichia pastoris with two antibiotic markers
    Article Snippet: Novel strain development pastoris.. Strains were first To determine if use of an increase copy number, the strain containing a differe that expressed both gelati cassettes.. Quantitative eva strated that increased exp both methods using two a

    Article Title: Gelatin expression from an engineered Saccharomyces cerevisiae CUP1 promoter in Pichia pastoris.
    Article Snippet: Funding information FibroGen, Inc Abstract The methylotrophic yeast Pichia pastoris (reclassified as Komagataella phaffii) is a versatile protein expression system, yet many commonly used promoters have attributes undesirable for fermentation or its optimization.. Hence, the copper-inducible CUP1 gene promoter from the related yeast Saccharomyces cerevisiae was used to express human gelatin.. Multimerization of a potential copper response element in the CUP1 promoter, a S. cerevisiae Ace1p binding site, significantly increased gelatin expression.

    Synthesized:

    Article Title: Distribution and potential biologic function of the thrombin receptor PAR-1 on human keratinocytes.
    Article Snippet: Thrombin has recently been shown not only to exert procoagulant activities, but also to induce mitogenic responses of different cell types involved in wound healing via binding to and cleavage of the thrombin receptor.. In order to further explore these aspects of thrombin function, human keratinocytes (HaCaT cell line) were examined for their potential mitogenic responsiveness to thrombin and for the dependency of this process on the expression of the high-affinity thrombin receptor.. Quiescent keratinocytes were stimulated in the mitogenic assay with α-thrombin and the thrombin receptor activating peptides TRAP42–55 (SFLLRNPNDKYEPY) and TRAP42–46 (SFLLR).



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